Figure 4 | Scientific Reports

Figure 4

From: Cultivated and wild Pleurotus ferulae ethanol extracts inhibit hepatocellular carcinoma cell growth via inducing endoplasmic reticulum stress- and mitochondria-dependent apoptosis

Figure 4

The effects of PFEE-C and PFEE-W on Δψm and intracellular ROS generation in H22 cells. H22 cells were treated with different concentrations of PFEE-C and PFEE-W. After 48 h, cells were stained with JC-1 and the fluorescence changes were observed using inverted fluorescence microscopy (a) and analyzed by flow cytometry (b). (c) After 24 h, proteins were isolated and the levels of Bax, Bcl-2 and cytochrome (cyto) c were detected by Western blot. Cropped blots are shown and full-length blots are included in the Supplementary Information. Grayscale scanning data were obtained by Image J. The ratios of Bax/Bcl-2 and cyto c/β-actin were shown in lower panels.

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