Figure 5

PFEE-C and PFEE-W activated caspases in H22 cells. H22 cells were treated with different concentrations of PFEE-C and PFEE-W. After 24 h, proteins were isolated and the levels of cleaved-caspases and -PARP were detected by Western blot. Cropped blots are shown and full-length blots are included in the Supplementary Information. Grayscale scanning data were obtained by Image J. The ratios of cleaved-caspases/caspases and cleaved-PARP/PARP were shown in lower panels. Data are from 3 independent experiments and analyzed by ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001 compared to untreated group.