Figure 5
Efficacy of Yhhu4952 in the cuprizone model. (a,b) Schematic representation of animal treatment. The C57BL/6 mice (n = 4 per group) were fed with 0.2% (w/w) of cuprizone containing diet for 6 weeks to induce demyelination. Following cuprizone withdraw, mice were treated with 10 mg/kg Yhhu4952 or normal saline for another 4 weeks. (c) Representative images (n = 6 images each from 4 mice per group) of corpus callosum region stained with Luxol fast blue and eosin after six weeks of cuprizone diet. (d,e) Quantifications of remyelinating areas. (f,g) Quantification of the relative MBP (green) fluorescence intensity in corpus callosum. Data are represented as mean ± SEM and analyzed using Student’s t test. *p < 0.05, **p < 0.01. Scale bar = 400 μm.
