Figure 7
Yhhu4952 promotes the differentiation of OPCs through the inhibition of Jagged1-Notch1 pathway. (a,b) Quantification of Notch1 protein expression after various concentration of Yhhu4952 treatment. (c) Real-time PCR demonstrating the Hes5 mRNA levels after various concentration of Yhhu4952 treatment. (d) Quantification of TGF-β1-induced Jagged1 expression levels. (e) Astrocyte-oligodendrocyte cocultures were stained with MBP (green) and GFAP (red). (f) Quantification of MBP+ cells per field. Data are expressed as mean ± SEM (three independent experiment were performed), and statistically analyzed using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001 compared with relative control. Full-length blots are presented in Supplementary Fig. 4e,f.
