Figure 2
From: Knock-down of AHCY and depletion of adenosine induces DNA damage and cell cycle arrest
Morphometric and cell cycle analysis. (A) Effects of AHCY silencing on the cell cycle distribution determined by flow cytometry after staining with propidium iodide. G1, S and G2/M indicate the cell phase in question. The graphs show the mean ± SD of three independent experiments performed in triplicate for each tested sample. (B) Changes in the cell proliferation rates of AHCY-silenced cells compared to control cells, as determined by NRA and MTT tests. The graphs show the mean ± SD of three independent experiments performed in at least 10 wells/cell line (* means P < 0.0.05; determined by two tailed t-test). (C) Upper panel: representative nuclei images (blue: DAPI). Centered is a field of view containing >10 cells for each condition. Graph: changes in the main nuclear morphometric features in % when compared with shCTRL set as 100% (mean of more than 100 nuclei; ** means p < 0.01 determined by two tailed t-test). Imaged with a Leica SP8 X FLIM confocal microscope (HC PL APO CS2 63 ×/1.40 OIL objective); scale bar, 50 µm.
