Figure 1

Differential gene expression of Tibetan hulless barley in response to powdery mildew. (A) Numbers of genes up-regulated (red) and down-regulated (green) in G7 and Z13 after inoculation with powdery mildew over time. (B) Heatmap of differentially expressed genes in G7. The columns show the 15 samples G7-0 hpi_1, G7-0 hpi_2, G7-0 hpi_3, G7-168 hpi_1, G7-168 hpi_2, G7-168 hpi_3, G7-6 hpi_1, G7-6 hpi_2, G7-6 hpi_3, G7-72 hpi_1, G7-72 hpi_2, G7-72 hpi_3, G7-36 hpi_1, G7-36 hpi_2, G7-36 hpi_3; while the rows show the TPM values scaled by the z-score algorithm. (C) Heatmap of differentially expressed genes in Z13. The columns show the 15 samples Z13-0 hpi_1, Z13-0 hpi_2, Z13-0 hpi_3, Z13-6 hpi_1, Z13-6 hpi_2, Z13-6 hpi_3, Z13-72 hpi_1, Z13-72 hpi_2, Z13-72 hpi_3, Z13-36 hpi_1, Z13-36 hpi_2, Z13-36 hpi_3, Z13-168 hpi_1, Z13-168 hpi_2, Z13-168 hpi_3; while the rows show the TPM values scaled by the z-score algorithm. Hierarchical clustering of expression pattern for genes in was shown at the left of the heatmap figure. (D) Clustering and classification of differentially expressed genes of G7 in response to Bgh over time (0, 6, 36, 72, 168 hpi). Twenty trends were determined, and the profiles of genes significantly enriched (P-value < 0.05) are colored. The number of genes in the trend is shown above each profile. (E) Clustering and classification of differentially expressed genes of Z13 in response to Bgh over time (0, 6, 36, 72, 168 hpi). Twenty trends were determined, and the profiles of genes significantly enriched (P-value < 0.05) are colored. The number of genes in the trend is shown above each profile.