Figure 3

FACS analysis of infiltrating immune cells in ConA-treated chimeric mice liver. (a–d) Liver immune cells were isolated 3 h after challenge with 25 mg/kg of ConA and subjected to FACS analysis (n = 3–9 per group). For the identification of total isolated immune cell populations, cells were stained with fluorophore-conjugated different extracellular marker proteins: CD45, CD11b, CD3ε, Ly6G, Ly6C, CD4, or CD8a. (a) Absolute numbers of total immune cells are shown. (b) Representative FACS images are shown. (c,d) The bar graph represents (c) the ratio of each cell population to the total CD45⁺ subset (%) and (d) the absolute number of each type of immune cells. Data are means ± SEMs. *P < 0.05 (Tukey-Kramer HSD test after the one-way ANOVA).