Figure 3
From: c-Fos induces chondrogenic tumor formation in immortalized human mesenchymal progenitor cells
c-Fos induces cytoskeletal changes and suppresses 3H cells invasion properties. (a) FACS plot representing cell size and complexity of transduced cells. (b) In vitro cell morphology after lentiviral transduction. (c) Representative immunofluorescence images of vimentin intermediate filament (Green: vimentin, Blue: DAPI) (n = 3). (d) Representative immunofluorescence images of Actin cytoskeleton (Red: Palloidin staining, Blue: DAPI) (n = 3). (e) RT-qPCR showing Tropomyosin 1 expression (n = 3). (f) Graphical representation and quantitative data of cell-displacement 17 hours after seeding at low density (data provided as mean euclidean distance displacement per cell) 10 cells are shown per condition (n = 10). (g) Transwell migrated cell-number quantification and representative images of crystal violet stained cells, after 24 hours of migration induction (n = 3). (h) Quantification and representative images of wound healing ability in cultured cells measured 12 hours after would formation (quantification is provided as cell-covered wound area) (n = 3). (3H-Ø, empty vector transduced cells; 3H-Fos, c-Fos vector transduced cells.) (unpaired t-test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).
