Figure 1 | Scientific Reports

Figure 1

From: Levels of Par-1 kinase determine the localization of Bruchpilot at the Drosophila neuromuscular junction synapses

Figure 1

Precise levels of Par-1 are required for BRP localization. (A) Representative confocal stacks showing axon bundles from third instar larvae of WT and Slmb mutant (slmb3A1). Axon bundles are stained with antibodies against BRP (Red), DVGLUT (Green) and HRP (Blue), n > 10, Scale bar = 10 μm. (B) Mean fluorescent intensity of BRP (BRP fluorescence normalized to HRP) in axon bundles represented in A. (C) Mean fluorescence intensity of DVGLUT normalized to HRP intensity (D) Representative confocal stacks showing axon bundles from third instar larvae of WT and Par-1RNAi. Axon bundles are stained with antibodies against BRP (Red), DVGLUT (Green) and HRP (Blue), n > 10, Scale bar = 10 μm. (E) Mean fluorescence intensity of BRP normalized to HRP intensity. (F) Mean fluorescent intensity of DVGLUT normalized to HRP intensity. Error bars represent S.E.M. N.S. = p > 0.05, **p ≤ 0.01, ****p ≤ 0.0001.

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