Figure 2

Accumulation of BRP in axons precedes its loss from synapses. (A,B) Representative axon bundles from larvae overexpressing Par-1 using GeneSwitch-Elav-Gal4. Time (T) represents time after exposure to RU-486 (T₀, T₂₄, T₄₈, and T₇₂) containing food. Axon bundles (A) are stained with antibodies against Par-1 (Green), BRP (Red) and HRP (Blue). Scale bar = 10 μm. Synaptic boutons (B) are stained with identical antibodies as A. Scale bar = 5 μm. (C) Mean Par-1 intensity normalized to HRP intensity within axons bundles (D) and mean BRP intensity normalized to HRP intensity in axon bundles (D) from same series of time points as A. n > 20. (E) Mean Par-1 intensity from synaptic boutons normalized to HRP (F) and quantification of the number of active zones (BRP puncta) per NMJ from same time points as B. n > 15. Error bars represent S.E.M. N.S. = p > 0.05, ^*p ≤ 0.05, ^**p ≤ 0.01, ^***p ≤ 0.001, ^****p ≤ 0.0001.