Figure 4

Futsch does not mediate accumulation of BRP within axons. (A) Representative confocal image stacks showing NMJ synapses from WT, Par-1OE and Par-1T408A third instar larvae stained with anti-Futsch (Red) and anti-HRP (Blue) antibodies. (B) Mean Futsch fluorescence intensity (A.U.) normalized to HRP intensity from entire NMJ arbor. n > 10. (C) Representative confocal stacks from larvae overexpressing Par-1 using GeneSwitch-ElavGal4 at T0, T24, T48, and T72. NMJ synapses are stained with anti-Futsch (Red) and anti-HRP (Blue) antibodies. (D) Mean Futsch fluorescent intensity (A.U.) normalized to HRP intensity from entire NMJ arbor. n > 20. Error bars represent S.E.M. N.S. = p > 0.05, **p ≤ 0.01.