Figure 1
CD86 and MHC II expression and cytokine production in murine dendritic cells stimulated with GXM or GalXM capsular polysaccharides. Dendritic cells (2 × 105/well) were incubated for 24 h in the presence of 50 μg/mL of capsular GXM or GalXM (wild-type strain B3501) from Cryptococcus neoformans or 10-ng/mL lipopolysaccharide (LPS) in the presence or absence of polymyxin B (100 ng/mL). Cells with labeled (A) CD11c (phycoerythrin; PE) and CD86 (FITC) or (B) CD11c (PE) and MHC II (FITC) were checked by flow cytometry along with positive cells. The control corresponds to dendritic cells without stimulus. The graph represents the mean fluorescence intensity (MFI). The result represents one of three independent experiments. The asterisks indicate ***P < 0.001, **P < 0.01, and *P < 0.05 compared to the control, and the hash symbol (#) indicates P < 0.01 compared to LPS without the addition of polymyxin B. To test cytokine production after stimulation, the culture supernatants were collected, and the production of cytokines IL-12p40 and IL-10 were determined with an ELISA assay. The control corresponds to dendritic cells without stimulus. The results represent one of four independent experiments. The asterisks indicate ***P < 0.001 and **P < 0.01 in relation to the control.
