Figure 4
CD4+ T cells in culture with murine dendritic cells pretreated with GalXM secrete IFN-γ. Dendritic cells (3 × 104/well) were incubated for 24 h in the presence of 50 μg/mL of the constituents’ capsule of C. neoformans. The dendritic cells were then incubated with CD4+ T cells (3 × 105/well) for 48 h. The culture supernatant was collected, and ELISA assays were used to determine the production of (A) IFN-γ and (B) IL-4. ConA was used at 5 μg/mL, and anti-IFN-γ and its IgG1a κ control were used at a concentration of 3 μg/mL. The control corresponds to dendritic cells without stimulus. The results represent one of four independent experiments. The asterisks indicate **P < 0.01 and *P < 0.05 relative to the control.
