Figure 5
Murine dendritic cells pre-treated with capsular GalXM in co-culture with CD4+ T cells induce the production of cytokines that direct the Th17 profile. Dendritic cells (3 × 104/well) were incubated for 24 h in the presence of 50 μg/mL of capsular GXM or GalXM from C. neoformans. The dendritic cells were then incubated with CD4+ T cells (3 × 105/well) for 48 h. The culture supernatant was collected, and the cytokines (A) TGF-β, (B) IL-6, and (C) IL-17 were analyzed with ELISA assays. The αCD3 and LPS were used at 5 μg/mL and 100 ng/mL, respectively. The control corresponds to dendritic cells without stimulus. The results represent one of four independent experiments. The asterisks indicate ***P < 0.001, **P < 0.01, and *P < 0.05 relative to the control.
