Figure 6

TRPA1 channels with 2 subunits harboring the C622S mutation can be activated by reactive electrophiles. (A,B) Activation by 100 µM cinnamaldehyde of currents in cells transfected with WT-D918H or WT-C622S/D918H. In both cases, cinnamaldehyde activated a current that could be inhibited by the TRPA1-specific blocker, A967079, and by Zn²⁺ (40 nM), reversibly. (C) Summary of current magnitudes measured before and after cinnamaldehyde treatment from experiments as in (A,B). **P < 0.01, ***P < 0.001 (Tukey’s multiple comparison following two-way ANOVA). (D) Inhibition of WT-C622S/D918H currents by the indicated concentrations of extracellular Zn²⁺. A pH 4 solution was used as a control; inhibiton of currents was qualitatively similar to that of WT-D918H currents. (E) Dose-dependence of inhibition by Zn²⁺ responses of WT-C622S/D918H currents (N = 5) is similar to that of WT-D918H. Data for WT, WT-D918H and D918H currents were replotted from Fig. 2. (F) Summary of recovery percentage (Rec %) at the wash-off of 40 nM Zn²⁺. n.s. P > 0.05 (unpaired t-test); N = 5.