Figure 5 | Scientific Reports

Figure 5

From: Low plasma haptoglobin is a risk factor for life-threatening childhood severe malarial anemia and not an exclusive consequence of hemolysis

Figure 5

Flow chart showing the variant filtering strategies and association studies used in the Ibadan HTS-Cohort. High throughput sequencing of Haptoglobin gene has been performed on 401 samples. We used an allele frequency filtering to select the variants with a MAF ≥ 10%. ANOVA and linear regression analysis were then used to evaluate their association with the haptoglobin level in CC group. One variant rs12162087 was selected upon this association. GenePop software was used to determine the Hardy Weinberg equilibrium in control group. We performed a binary logistic regression to test the association of this variant and SMA. The allele and the genotype frequencies for rs12162087 are indicated for each disease group. (UM) = Uncomplicated Malaria; (SMA) = Severe Malarial Anemia; (CM) = Cerebral Malaria; (CC) = healthy malaria-negative Community Controls; (N) = number of subjects; MP(+ve) = malaria parasite positive; MP(−ve) = malaria parasite negative, MAF = Minor allele frequency. For HTS Cohort clinical group details see Table 1 and details for variants see Table 2.

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