Figure 5
From: T cell-derived lymphotoxin limits Th1 response during HSV-1 infection
Blocking the LT-LTβR signaling promotes infiltration of monocytes and monocyte-derived DCs in the draining lymph node. (a) MCP-1 secretion in the popliteal LN from WT mice (solid line) and LTβR-Ig-treated mice (dotted line) detected by CBA test (n = 5/group). (b) Gating strategy of monocytes and moDCs, shown by representative dot plots from day 4 p.i. (c) Absolute number of monocytes in the popliteal LNs (n = 4/group). (d) Absolute number of moDCs in the popliteal LNs (n = 4/group). (e) Monocytes, moDCs and non-monocyte-derived DCs (CD11c+CD11b−Ly6C−MHC-II+) were purified on day 4 p.i. Expression of IL-12 was analyzed by RT-PCR (n = 3/group). Data are representative of three independent experiments, shown as mean ± SEM. n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001. (Two-way ANOVA multiple comparisons for (a); two-tailed paired Student’s t test for c–e).
