Figure 1 | Scientific Reports

Figure 1

From: Astragaloside IV inhibits glucose-induced epithelial-mesenchymal transition of podocytes through autophagy enhancement via the SIRT–NF-κB p65 axis

Figure 1

AS-IV effects on hyperglycaemia-triggered podocyte EMT. (A) AS-IV chemical structure. (B–E) Podocytes were pretreated with high/normal glucose for 1 hour, and then incubated with or without AS-IV (25, 50 and 100 μM) for 48 hours. (B) mRNA expression levels of TGF-β were detected using real-time PCR. (C) TGF-β protein levels were quantified using Western blotting. (D) mRNA expression levels of α-SMA, N-cadherin, E-cadherin and nephrin were detected using real-time PCR. (E) Protein levels of α-SMA, N-cadherin, E-cadherin and nephrin were detected using Western blotting. Note: E-Ca, E-cadherin; N-Ca, N-cadherin. The molecular weight of the proteins: TGF-β, 44 kDa; nephrin, 100 kDa; E-cadherin, 110 kDa; N-cadherin, 100 kDa; α-SMA, 42 kDa. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort or AS-IV cohort, P < 0.05; #compared with high glucose cohort, P < 0.05.

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