Figure 3

Loss of FGF21 signaling to adipose tissues does not affect core body temperature during cold exposure. (A) Liver, (B) BAT, (C) iWAT and (D) eWAT Klb mRNA levels in 11–13 week old male wild type (WT) and KLB AdipoKO mice housed at thermoneutrality or following 3 days of cold exposure (n = 6–7/group). (E) Core body temperature (°C) and heat production (kcal/h) during thermoneutral and cold phases of 11–13 week old male KLB AdipoKO mice relative to WT controls (n = 6–7/group). Heat production by hour is estimated by the modified Weir equation. Core body temperature (°C) is also plotted against change in heat production (kcal/h). Change in core body temperature and heat production relative to time of day is also presented for KLB AdipoKO mice compared to WT controls (n = 6–7/group). Values are mean ± SEM; ^*P ≤ 0.05; ^**P ≤ 0.01; ^***P ≤ 0.005; and ^#P ≤ 0.001 compared to wild type mice.