Figure 6 | Scientific Reports

Figure 6

From: Ghrelin protects against contact dermatitis and psoriasiform skin inflammation by antagonizing TNF-α/NF-κB signaling pathways

Figure 6

Ghrelin exerts its anti-inflammatory effects on contact dermatitis and psoriasis through NF-κB signaling. (A) Expression of mRNA NF-κB2 is suppressed upon treatment with ghrelin in an OXA-treated contact dermatitis mouse model. (B,C) The significantly increased p-IκBα in response to stimulation by OXA was markedly inhibited by ghrelin, as shown by western blotting. (D) Expression of p-IκBα in ear samples was significantly enhanced by OXA stimulation, while ghrelin decreased p-IκBα levels, as shown by immunohistochemistry. (E) mRNA levels of NF-κB2 are markedly decreased by ghrelin treatment in an IMQ-induced psoriasis mouse model, as shown by real-time PCR. (F,G) Significantly decreased expression of p-IκBα in the total protein extracted from skin tissue was detected after application of ghrelin in the IMQ-induced psoriasis mouse model, as detected by western blotting. (H) p-IκBα is significantly decreased upon treatment with ghrelin in the psoriasis mouse model, as shown by immunohistochemistry. (*p < 0.05, **p < 0.01, ***p < 0.005 vs the control group). Scale bar: 100 μm.

Back to article page