Figure 4 | Scientific Reports

Figure 4

From: Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9′-methyl-3′-carbazole)-3, 4-dihydro-β-carboline

Figure 4

Hydrogen-deuterium exchange (HDX) assays of MIF in the presence and absence of MCDC. The deuterium uptake curves for six representative peptides are shown. Numbers in parentheses are the amino acid positions of each peptide. HDX profiles of MIF protein (1.5 pmol) in the absence (black dot) and presence (white dot) of MCDC (150 pmol) show how many deuteriums were incorporated. The y-axis is the amount of deuterium that could be incorporated to each peptide. A native detergent (0.13% of MMPC, 1-myristoyl-sn-glycero-3-phosphocholine) was used to dissolve the MCDC. The molar ratio of MIF:MCDC equals 1:100 (white dot) or 1:0 (black dot) in 0.13% of MMPC.

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