Figure 1
From: Extensive reprogramming of the nascent transcriptome during iPSC to hepatocyte differentiation
Protein-coding transcripts in estimating the hepatic differentiation. (A) Heatmap representation of the protein-coding genes in iPSCs, PHHs, M1- and M2-HLCs (FPKM > 1 in at least one of the samples; logFC > 2 and adj. p-value < 0.05 for at least one comparison). Unsupervised hierarchical cluster analysis was performed on differentially expressed protein-coding genes between iPSCs, PHHs and HLCs. A dendrogram demonstrates similarity and heatmap illustrates gene expression changes between the samples. Different samples are listed in columns and genes in rows. (B) Venn diagrams of differentially expressed protein-coding genes when comparing PHHs/M1-HLCs/M2-HLCs to iPSCs. (C) Gene ontology (GO) analyses of the differentially expressed protein-coding genes in M1-HLCs, M2-HLCs and PHHs vs. iPSCs. (D) Heatmap representation of a restricted set of genes related to pluripotency, hepatic differentiation and liver functions. (E) A bar chart representation of 21 cytochrome P450 (CYP) enzymes that were differentially expressed between the M1- and M2-HLCs (adj. p-value < 0.05). See also Supplementary Fig. S5.
