Figure 4
From: Extensive reprogramming of the nascent transcriptome during iPSC to hepatocyte differentiation
Changes in regulatory motifs reflect the altered repertoire of transcription factors (TFs). (A) Sequence motifs associated with lncRNAs for up- and down-regulated transcripts in PHHs vs. iPSCs. (B) Heatmap of normalized RPKM values (−1 to 1) of 32 differentially expressed TFs during hepatocyte differentiation for which motif information existed. Clustering was performed for genes and samples using Spearman’s rank correlation (complete linkage). (C) Heatmap of normalized motif enrichment percentages (−1 to 1) of the TFs listed in (B). (i) The down-regulated transcripts being enriched for potential transcriptional repressors, (ii) the up-regulated transcripts enriched for potential transcriptional repressors, (iii) the up-regulated lncRNAs transcripts being enriched for the binding of several hepatocyte-specific TFs.
