Figure 3
From: Low density lipoprotein mimics insulin action on autophagy and glucose uptake in endothelial cells
LDL mimics the role of insulin on autophagy. HUVECs were starved by culture in serum-free medium and incubated with LDL (50 μg/mL, 30 min) or insulin (100 nM, 20 min). Cell lysates were analyzed using Western blots. The expression of autophagy-associated proteins, and PI3K/Akt/mTOR and PI3K/Akt/GSK3β pathway proteins were analyzed. The expression in Ctr group cells was assigned the value of 1, n = 3, *p < 0.05, **p < 0.01 versus Ctr. #p < 0.05, ##p < 0.01 versus LDL. Data expressed as mean ± S.E.M.
