Figure 2

Effect of pressure overload on LV cardiomyocytes and calcium handling. (A) Cardiomyocytes active tension; (B) myofilaments calcium sensitivity; (C) Hill-coefficient; (D) the rate of tension redevelopment (Ktr); protein quantification of: (E) sarcoplasmic/endoplasmic reticulum Ca²⁺ATPase 2a (SERCA2a); (F) ratio of SERCA2a to phospholamban; (G) sodium-calcium exchanger; (H) ratio of phosphorylated to total Ca²⁺/calmodulin-dependent protein kinase (CAMKii); mRNA expression of calcium-binding protein A1 (S100A1); (J) representative western blot lanes. For representative purposes the image was cropped and edited; (K) representative membrane protein loading. Values are mean ± SEM. One Way-ANOVA: Ba/Deb vs Sh: ^αp < 0.05; ^ααp < 0.01; ^αααp < 0.001; Deb vs Ba: ^χp < 0.05; ^χχp < 0.01, ^χχχp < 0.001.