Figure 4
Effect of pressure overload on RV cardiomyocytes and calcium handling. (A) cardiomyocytes passive tension; (B) cardiomyocytes active tension; (C) myofilaments calcium sensitivity; (D) Hill-coefficient; (E) the rate of tension redevelopment (Ktr); Protein quantification of: (F) sarcoplasmic/endoplasmic reticulum Ca2+ATPase 2a (SERCA2a); (G) ratio of phospholamban phosphorylated (p-PLB) to total (PLB); (H) ratio of SERCA2a to phospholamban; (I) sodium-calcium exchanger (NCX); (I) Junctophilin; (L) representative western blot lanes (for representative purposes the image was cropped and edited) representative membrane protein loading. n = 5 for each group. Values are mean ± SEM, One-way ANOVA. Ba/Deb vs Sh: αp < 0.05; ααp < 0.01; αααp < 0.001; Deb vs Ba: χp < 0.05; χχχp < 0.001.
