Figure 6
From: Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function
The effect of loss of LIMKs on cofilin. (a) Western blot was performed to detect the expression level of cofilin and p-cofilin in both P30 WT and DKO mice, n = 3. (b) Quantification of the Western blot (p < 0.01). (c) Auditory HCs of P30 DKO and WT mice were stained with cofilin and p-cofilin antibodies. Images were taken from the basal turn of the cochlea, and there was no difference from the apical to basal turns. Scale bar = 10 µm. (d) Quantification of cofilin and p-cofilin immunolabeling in the basal turns of OHCs (p < 0.05) n = 3. (e) RT-qPCR was performed with P30 WT and DKO mice cochleae (p < 0.05, n = 5). Data are presented as mean ± SD.
