Figure 1

Differential expression of host lncRNAs after expression of HPV-16 E6 in primary foreskin keratinocytes. (A) Waterfall plot of host lncRNAs altered 1.5-fold or greater in primary foreskin keratinocytes (HEKa) expressing HPV-16 E6 compared to HEKa expressing GFP by high-throughput RNA sequencing (RNA-seq) analysis. Triplicates for each sample were sent for RNA-seq. (B) qRT-PCR validation of representative differentially expressed host lncRNAs found by RNA-seq analysis. Red bars represent the lncRNAs up-regulated with HPV-16 E6 expression, and green bars represent the lncRNAs down-regulated with HPV-16 E6 expression. GAPDH mRNA was used to normalize the qRT-PCR analyses, which are shown relative to uninfected HEKa (grey bars).