Figure 4
FAM83H-AS1 is localized in the nucleus in cervical pre-malignant and cancerous cell lines. (A) qRT-PCR of FAM83H-AS1 expression in fractionated HPV-16 positive cervical cancer CaSki (A) and pre-malignant W12/201402 (B) cell lines. U6 small nuclear RNA (snRNA) was used as a nuclear control RNA and mature Beta Actin was used as a cytoplasmic control RNA. Representative images; similar results were obtained in at least three independent experiments. Normalization was done using C. Elegans total RNA as an exogenous spike for the amplification of worm-specific ama-1 gene. Two-tailed t test results are indicted as **p ≤ 0.01.
