Figure 4
Hormonal stimulation significantly increases levels of DUOX, but does not elevate basal ROS production in GCs. Results of qRT-PCR experiments showed that mRNA expression levels of NOX5/DUOX1/2 were elevated after hormonal stimulation, but not all reached statistical significance (A,B). Results (mean ± SEM) shown were normalized to the geometric mean of the reference genes RPL19, UBC, PPIA and GAPDH. (C) Measurements of ROS production using H2DCFDA did not show any difference after 2 h measurement when treated with the hormones. Numbers of repeated experiments are shown inside the columns. Mean ± SEM; one-sample t-tests (two-tailed; *p < 0.05)
