Figure 3
From: Beta cell secretion of miR-375 to HDL is inversely associated with insulin secretion
Stimulation of insulin secretion blocks INS-1 export of miR-375-3p to HDL. (a) miR-375-3p levels on cf-nHDL and INS-1-nHDL after INS-1 cell treatment with 3 mM or 11 mM D-glucose in the presence or absence of 1.8 mM CaCl2. n = 3–6; mean ± 95% CI; One-way ANOVA with Bonferroni post-test, alpha = 0.05. (b) HDL-miR-375-3p levels on cf-nHDL and INS-1-nHDL from INS-1 cells treated with tolbutamide for 2 h. n = 7–9; mean ± 95% CI; One-way ANOVA with Bonferroni post-test, alpha = 0.05. (c) miR-375-3p levels on cf-nHDL and INS-1-nHDL after INS-1 cell treatment with vehicle or diazoxide (DIA) in the presence or absence of 1.8 mM CaCl2. n = 3; mean ± 95% CI; One-way ANOVA with Bonferroni post-test, alpha = 0.05.(d) miR-375-3p levels on cf-nHDL and islet-nHDL from mouse WT (wildtype) or SUR1 KO (Abcc8−/−) mice. n = 3; mean ± 95% CI; One-way ANOVA with Bonferroni post-test, alpha = 0.05. (e) HDL-miR-375-3p levels on cf-nHDL and INS-1-nHDL from INS-1 cells treated with exendin-4 (ex-4) or IBMX for 3 h. n = 3; mean ± 95% CI; One-way ANOVA with Bonferroni post-test, alpha = 0.05. (f) Schematic depicting miRNA export to HDL in low glucose (left panel), and suppression of export in high glucose (right panel).
