Figure 4

Beta cell miR-375-3p export to HDL does not require cholesterol transporters. (a) miR-375-3p levels on cf-nHDL and islet-nHDL from mouse WT (wildtype) or SR-BI KO (Scarb1^−/−) mice. n = 3; mean ± 95% CI; One-way ANOVA with Bonferroni post-test, alpha = 0.05. (b) INS-1 cellular levels of Scarb1 mRNA and (c) SR-BI protein (western blotting) after transfection with mock or 50 nM siRNA against Scarb1. Cropped images are of the same blot at different exposures. Full blots are available in Fig. S10. n = 12; mean ± 95% CI; One-way ANOVA with Bonferroni post-test, alpha = 0.05. (d) miR-375-3p levels on cf-nHDL and INS-1-nHDL after knockdown of Scarb1; cells were transfected with mock or 50 nM Scarb1 siRNA. n = 6; mean ± 95% CI; One-way ANOVA with Bonferroni post-test, alpha = 0.05. (e) ABCA1 and (f) ABCG1 protein (western blotting) after transfection with mock or 50 nM siRNA against Abca1 and Abcg1, in the presence or absence of TO901317 (LXR agonist) and 9-cis-retinoic acid (RXR agonist). Representative of n = 3. Cropped images are of the same blots at different exposures. Full blots are available in Fig. S10. (g) miR-145-5p and miR-375-3p levels on cf-nHDL and INS-1-nHDL after INS-1 cell transfection with mock or 50 nM siRNAs against Abca1 and Abcg1 and/or LXR/RXR agonists. n = 6; mean ± 95% CI; One-way ANOVA with Bonferroni post-test, alpha = 0.05.