Figure 5
Undifferentiated hiPSC transcriptome profiling using CAGE. (a) Scatter plot shows fold changes of individual genes from CAGE. (b) Graph showing the fold changes of the top and bottom differentially expressed promoters according to CAGE comparing the high differentiation group with the low differentiation group. Selected genes have been color-coded and labelled. Red, top 10 expression in the high differentiation group; blue, top 10 expression in the low differentiation group (FDR (false discovery rate) < 0.01, FC > 2). (c) Pathway analysis of the collective expression levels of interacting genes involved in specific pathways. (d) List of predictive biomarkers for cardiac differentiation. Venn diagram analysis visualising the overlap among the candidate genes identified using CAGE, mRNA array, and miRNA array analyses. The number of upregulated and downregulated mRNAs in hiPSC lines of the high differentiation group compared to that in the low differentiation group is indicated by red (up) and blue (down) colours, respectively.
