Figure 6
Validation results for expression of 22 genes in a test set of 13 hiPSC lines. (a) Percentage of cTnT-positive cells generated from hiPSCs in high and low differentiation groups at 17 d post-differentiation in a test set of hiPSC lines. Data are expressed as mean ± SEM (n = 3–10). **p < 0.01, t-test. (b) PF4 and TMEM64 mRNA expression levels in the high and low differentiation groups were quantified using qRT-PCR. Data are expressed as mean ± SEM (n = 3–10). **p < 0.01; *p < 0.05, t-test. (c) PF4 and TMEM64 mRNA levels in undifferentiated hiPSCs correlated with their cardiac differentiation efficiency along with r and p values. All mRNA values are shown as fold change relative to the expression of PCi-1533. Each dot indicates the expression level in each hiPSC line. (d) Percentage of cTnT-positive cells generated from hiPSCs with a common genetic background (253G1) incubated with IWR-1 & IWP-2, CHIR99021, and Mitoblock-6 at 17 d post-differentiation. Data are expressed as mean ± SEM (n = 6). **p < 0.01vs. DMSO, ANOVA and Dunnett’s test. (e) PF4 and TMEM64 expression levels of the hiPSCs incubated with IWR-1 & IWP-2, CHIR99021, and Mitoblock-6. All mRNA values are shown as fold change relative to the expression of mRNA in DMSO-treated control cells. Data are expressed as mean ± SEM (n = 6). *p < 0.05 vs. DMSO, ANOVA and Dunnett’s test.
