Figure 9
Effects of icariin on protein expression. MCF-7 cell lines were incubated without or with Icariin at 10 μM, 30 μM and 100 μM for 24 h. The proteins of cells were extracted and determined by western blotting. The pictures of BCl-2 (A), bax (A), parp (C) and p-Akt (D) were taken. Then protein pictures were analyzed with software. The Bax (B), p-Akt (D), BCl-2 (E), parp (F) and p-Akt (H) protein expressions were obtained and ratios of Bax to BCl-2 were calculated (F). Each experiment was done in triple. (The KDA of Parp is about 116 and the KDA of p-akt is about 60. After blocking, the membrane was cut on 70 kDa, actually these two proteins come from one gel, Therefore, the control in (C,D) was same.
