Figure 2
From: Maf deficiency in T cells dysregulates Treg - TH17 balance leading to spontaneous colitis
In vitro differentiation of Maf-deficient CD4+ T cells in RORγt+ Treg is defective. CD4+ T cells isolated from splenocytes of Maff/f and MafΔTcells mice were cultured for 5 days in TH1 (IL-12 + anti-IL-4), TH2 (IL-4 + anti-IFN-γ), Treg (TGF-β +IL-2) or TH17 (TGF-β +IL-6) polarizing conditions. (A) Representative histograms for the expression of master transcription factors associated with each helper subset: TBET (TH1), GATA3 (TH2), FOXP3 (Treg) and RORγt (TH17 FOXP3+ and FOXP3-) from Maff/f and MafΔTcells mice. (B) Percentage of each master transcription factor according to the condition of polarization among CD4+ T cells (C) Representative histograms for the expression of MAF in in vitro differentiated CD4+ T cell subsets according to the expression of their master transcription factor from Maff/f and MafΔTcells mice. MFI is indicated for T cells from Maff/f (grey) or MafΔTcells (black) mice. (D) Percentage of MAF+ cells among each TH subset according to the expression of their master transcription factor from WT mice. Each symbol represents an individual mouse. Data are representative of 2 independent experiments with at least 2 mice per group. All graphs indicate means. Error bars display SEM.
