Figure 3

Maf^ΔTcells mice are deprived of RORγt⁺ T_reg. (A) Quantitative RT–PCR of colonic tissues from Maf^f/f (n = 6) and Maf^ΔTcells (n = 8) mice for the indicated transcripts. Gene expression levels were normalized to Beta2microglobulin. (B) Representative histogram of the expression of MAF in mLN (left) and percentage of MAF⁺ cells (right) in mLN and colon in RORγt⁻ T_reg, RORγt⁺ T_reg and T_H17 cells from Maf^f/f mice (n > 10). (C) Representative contour plot of the expression of FOXP3 and RORγt in CD4⁺ T cells from spleens, mLN and colons of Maf^f/f and Maf^ΔTcells mice. (D) Percentage of RORγt⁻ T_reg, RORγt⁺ T_reg and T_H17 cells from spleens, mLN and colons of Maf^f/f and Maf^ΔTcells mice (n > 10 per group). (E) Percentage of RORγt⁻ T_reg, RORγt⁺ T_reg and T_H17 cells in CD4⁺ T cells from colons of Maf^f/f and Maf^ΔTcells mice at indicated ages (n > 4 per group). (F) Percentage of MAF⁺ cells among RORγt⁻ T_reg, RORγt⁺ T_reg and T_H17 cells from mLN and colons of Maf^f/f and Maf^ΔTcells mice at indicated ages (n > 4 per group). (A–C) Each symbol represents an individual mouse. Data are representative of at least 2 independent experiments with at least 3 mice per group. (E,F) Data are representative of at least 2 independent experiments with at least 2 mice per group. All graphs indicate means. Error bars display SEM.