Figure 6
From: Maf deficiency in T cells dysregulates Treg - TH17 balance leading to spontaneous colitis
IL-10 expression is regulated by MAF in Treg and TH17 cells. (A) Quantitative RT–PCR of colonic tissues from Maff/f (n = 3), MafΔTcells (n = 4) and MafΔTreg (n = 5) mice for the indicated transcripts. Gene expression levels were normalized to Beta2microglobulin. (B) Quantitative RT–PCR of isolated Treg (CD4+CD25+CD45RBlo) of spleens and LN of Maff/f (n = 5), MafΔTcells (n = 6) and MafΔTreg (n = 6) mice for the indicated transcripts. Gene expression levels were normalized to Beta2microglobulin. (C) Representative contour plot of the expression of IL-10 and RORγt in Treg from mLN of Maff/f, MafΔTcells and MafΔTreg mice. (D) Percentage of IL-10+ cells among RORγt- (left) and RORγt+ Treg (right) in mLN and colon of Maff/f, MafΔTcells and MafΔTreg mice after ex vivo restimulation (n > 8 per group). (E) Percentage of IL-10+ cells among TH17 cells from mLN of Maff/f, MafΔTcells and MafΔTreg mice after ex vivo restimulation (n > 5 per group). (F) Percentage of IFN-γ+ and IL-17A+ cells among TH17 cells from colons of Cre-negative littermates, MafΔTcells and MafΔTreg mice after ex vivo restimulation (n > 6 per group). (A,B,D–F) Each symbol represents an individual mouse. Data are representative of 2 independent experiments. All mice were over 20 weeks old. All graphs indicate means. Statistical significances were calculated by a Tukey test after a significant one-way ANOVA.
