Figure 2

Effects of sample fixation on the retention of proteins of the method using PVDF membranes. (a) Indicated numerals are amounts (5.0, 2.5, 1.3, and 0.6 μg) of the pooled serum proteins were subjected to 10% SDS-PAGE. The blotted membranes were treated using the traditional (left panel) or optimised fixation protocol (right). (b) Staining intensities were statistically analyzed (n = 3 individual experiments). Solid bar, no fixation; White bar, optimised fixation protocol. The exposure times were the same in all procedures. Band intensities were analysed and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. ^*Significantly different p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. All values are means ± S.E. (error bars).