Figure 2 | Scientific Reports

Figure 2

From: Seed targeted RNAi-mediated silencing of GmMIPS1 limits phytate accumulation and improves mineral bioavailability in soybean

Figure 2

Linear and circular map of pBIN-MIPS AS. MIPS1 ORF (~1.5 kb) region introduced in antisense orientation under vicilin promoter at BamHI/XbaI site. Restriction digestion of pBIN-MIPSAS; Lane1: λ-EcoRI/HindIII marker, Lane2: BamHI/XbaI restriction showing ~12.5 kb binary vector fragment and ~1.5 kb MIPS1 antisense fragment, Lane3: PCR amplification of MIPS1 from pBIN-MIPS AS, Lane4: 1 kb ladder.

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