Figure 3
From: Stress-induced tunneling nanotubes support treatment adaptation in prostate cancer
Disruption of TNT formation sensitizes PCa cells to AR blockade and metabolic stress. (A) LNCaP cells were treated with androgen deprivation (CSS) combined with TNT disruption by gentle shaking (250 rpm) or actin inhibitor Cytochalasin D and TNT formation was quantified. ***p < 0.001. (B,C) Cell survival of LNCaP cells after combined treatment with CSS and TNT-disruption was assessed by crystal violate (c.v.) staining (B, **p = 0.001, ***p < 0.001) or automated cell counter (C, *p = 0.024, ** p = 0.005). (D,E,F) PC3 cells were treated with SS in combination with TNT-disruption, and TNT formation and cell survival estimated as described for LNCaP cells. *p = 0.002 for shaking, and **p = 0.007 for Cytochalasin D. ***p < 0.001 compared to ctrl. All data is presented as average +/− SD from 3 independent experiments.
