Figure 3
Fbxo41 promotes disassembly of primary cilia. (a) Typical examples of images used to quantify cilia length. (b) Primary hippocampal neurons were infected with the indicated constructs at DIV1 or 13, and fixed at DIV15 or 21, and immunostained with GFP (green), ACIII (red) and MAP2 (blue) antibodies. (c) Immunoblot of primary cortical neurons infected with the indicated constructs at DIV1 and lysed at DIV16. Manipulating Fbxo41 expression does not alter ACIII or Arl13b expression levels. Note the diffuse ACIII band is caused by variable glycosylation63,64. Gel was cropped for clarity (full length blot available in Supplementary Fig.Ā S5). (d) Percentage of ciliated neurons infected at DIV 1 and fixed at DIV 15. Fbxo41 accumulation, but not depletion, significantly affects the percentage of ciliated neurons. Data from three independent experiments. (e) Quantification of cilia length from neurons in (d). (f) Cilia length in neurons infected with the indicated constructs at DIV 13 and fixed at DIV21. Fbxo41 promotes cilia disassembly rather than inhibiting ciliogenesis. Data from two independent experiments.
