Figure 5

Phorbol esters, but not neuronal activity, promote ciliary disassembly. (a) In neurons at DIV 16 cilia length is unchanged by decreasing (using APV & DNQX or TTX) or increasing neuronal activity (using Gbz), but it is significantly lower in neurons treated with Phorbol ester (PDBU). Data from five independent experiments. (b,c) At DIV 16 the decrease in cilia length caused by 6 h treatment with PDBU correlates with an increase in centrosomal accumulation of Fbxo41. Data from two independent experiments. (d) Neurons treated at DIV 11 with PDBU also show a decrease in ciliary length. This decrease is also observed in neurons lacking Fbxo41 (expressing shRNA against Fbxo41 since DIV1). **p < 0,01 ****p < 0,0001 Kruskal-Wallis Test, followed by a post-hoc Dunn’s multiple comparisons test. Data are represented as mean ± SEM.