Figure 1

Inhibition of the purinergic pathway with oATP reduces corneal infiltration of leukocytes and suppresses APC maturation. (A) Schematic diagram depicting the time points of corneal transplantation, oATP administration and tissue harvesting. (B) Representative flow cytometric dot plots showing the gating strategy for, and frequencies of, CD45⁺ inflammatory cells in the corneas of oATP-treated mice, relative to saline-treated and naïve mice. (C) Representative flow cytometric dot plots (left) showing the gating strategy for selecting CD11b⁺MHCII⁺ cells in the cornea. Bar chart (right) summarizes the frequencies of CD11b⁺MHCII⁺ cells in the corneas of oATP-treated mice at 14 days after transplantation, relative to saline-treated mice. (D) Representative flow cytometric dot plots (left) and bar chart (right) depicting the frequencies of CD11b⁺MHCII^hi cells in the draining lymph nodes of oATP-treated mice, relative to saline-treated and naïve mice. n = 5–7/group. Representative data from three independent experiments are shown, and data are depicted as mean ± SEM. *p < 0.05; **p < 0.01.