Figure 2 | Scientific Reports

Figure 2

From: Use of syngeneic cells expressing membrane-bound GM-CSF as an adjuvant to induce antibodies against native multi-pass transmembrane protein

Figure 2

Establishment of membrane-bound cytokines on BALB/3T3 as cell adjuvants. (A) Schematic representation of membrane-bound cytokines constructs, which code for an HA epitope (HA), the murine cytokines (IL-2, IL-18, or GM-CSF), a myc epitope (Myc), and the transmembrane ___domain of murine B7-1 (B7 Tm). (B) Expression of membrane-bound cytokines in the transduced BALB/3T3. mIL-2, mIL-18, and mGM-CSF were detected by western blotting using anti-HA tag Ab. β-actin was used as the internal control of each cell lysate. Full blots are shown in Supplementary Fig. 1. (C) Surface expression of membrane-bound cytokines on the transduced BALB/3T3. mIL-2, mIL-18, and mGM-CSF were detected by flow cytometry after staining the cells with anti-IL-2 Ab, anti-IL-18 Ab, and anti-GM-CSF Ab, respectively.

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