Figure 4 | Scientific Reports

Figure 4

From: Use of syngeneic cells expressing membrane-bound GM-CSF as an adjuvant to induce antibodies against native multi-pass transmembrane protein

Figure 4

Characterization of anti-EVI2B Ab responses in immune serum. (A) Expression of EVI2B in the transient transfected cell adjuvants. EVI2B and each cytokine were detected by western blotting using anti-EVI2B Ab and anti-HA tag Ab, respectively. β-actin was used as the internal control of each cell lysate. Full blots are shown in Supplementary Fig. 2. (B) Surface expression of EVI2B in the transient transfected cell adjuvants. EVI2B was detected by flow cytometry after staining the cells with anti-EVI2B Ab. (C) BALB/c mice were s.c. injected with EVI2B-expressing cell adjuvants (3T3/EVI2B, 3T3/mIL-2/EVI2B, 3T3/IL-18/EVI2B, and 3T3/mGM-CSF/EVI2B). Anti-EVI2B Ab responses in mouse serum were measured by cell-based ELISA using 293 A/EVI2B cells (n = 9). Immune serum was 1000-fold diluted. P values were determined by Student’s Newman-Keuls test: *, P < 0.05.

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