Figure 5 | Scientific Reports

Figure 5

From: Use of syngeneic cells expressing membrane-bound GM-CSF as an adjuvant to induce antibodies against native multi-pass transmembrane protein

Figure 5

Anti-CXCR2 Ab responses in mice immunized with 3T3/mGM-CFS/CXCR2 cells. (A) Surface expression of CXCR2 and mGM-CSF on the 3T3/mGM-CSF/CXCR2 cells was determined by a commercial anti-CXCR2 Ab and anti-HA tag Ab via flow cytometry. (B,C) Anti-CXCR2 Ab responses in mouse serum. Four BALB/c mice were s.c. injected with 3T3/mGM-CSF/CXCR2 cells. Mouse serum were collected from BALB/c mice before (pre-immunization serum) and after immunization (immune serum). (B) Anti-CXCR2 Ab responses were determined by cell-based ELISA using 293 A/CXCR2 cells. Immune serum was 100- to 25600-fold diluted. Data are shown as mean ± SD. n = 3 biological replicates. (C) anti-CXCR2 Ab responses in mouse serum (immune serum-4) were determined by flow cytometry after staining the 293 A/CXCR2 or 293 A cells with mouse serum (100- to 900-fold dilution). Results represent the surface mean fluorescence intensities (MFI).

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