Figure 6
Evidence for replication fork rescue by converging replication in mammalian mitochondria. Interpretation of the molecule below each image. Arrowheads indicate replication forks. (A) The 72 h ddC treatment results in accumulation of replicating molecules in regular HEK293T cells, with the majority appearing as conventional replication bubbles. (B) A molecule containing a broken replication intermediate (orange) in addition to a replication bubble. (C) PvuII -digested mtDNA from the same cells showing a linearized molecule with a replication bubble. Assuming the ends to match the PvuII cut sites (see Fig. 3D), the replication forks map to OH and OL respectively. (D) Replication bubble containing an additional broken replication intermediate. Scale bar 1 µm. (E) Replication bubble from replication initiating outside of OH on the same molecule with a broken intermediate. Scale bar 1 µm.
