Figure 5
From: Elevated numbers of PD-L1 expressing B cells are associated with the development of AIDS-NHL
CD40L+ HIV induces PD-L1 expression on B cells, as well as IL10 secretion. Isolated B cells were exposed to mock supernatants, anti-CD40 (positive control) CD40L+ HIV and T147N-HIV (CD40L mutant-HIV) for 2 hours and then cultured at 37 °C for 3 days. (A) flow cytometry for PD-L1 was performed on cultured B cells and % of PD-L1+ B cells are shown, (B) IL10 levels in supernatants of cultured cells, measured using Luminex multiplexed assays. p-values were calculated using a Wilcoxon test.
