Figure 1
From: AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells
AAV GCG-EGFP leads to α-cell EGFP expression. Pancreas sections from adult C57BL/6 mice treated with AAV GCG-EGFP by (A) single intraperitoneal injection of 1012 vg, (B) single intraperitoneal injection of 1013 vg, and (C) single intraductal injection of 1012 vg. Glucagon (red), GFP (green), and DAPI (grey). (D) Quantification of cells with colocalization of both GFP and GCG as a ratio of total GCG-positive cells, from pancreas sections. (E) Small intestine section and (F) brainstem section at the level of the solitary tract nucleus, stained for GLP-1 (red), GFP (green), and DAPI (grey), from a mouse treated with 1013 vg of AAV GCG-EGFP by single intraperitoneal injection. Non-specific GFP staining can be seen in the lumen of the central canal in brainstem sections. Scale bars = 100 μm. AAV: adeno-associated virus; GCG: glucagon; GFP: green fluorescent protein; GLP-1: glucagon-like peptide 1.
